前庭単離有毛細胞の運動性について
1992 年 51 巻 Suppl-8 号 p. 95-99
詳細
1992 年 51 巻 Suppl-8 号 p. 95-99
Vestibular hair cells were isolated from the saccular and utricular macula, or crista ampul-laris of the guinea pig by enzymatic and mechanical dissociation. The isolated cells were classified into three types ; flask-shaped type I cells, rod-shaped type II cells and round suppo rting cells. The cilia of type I in the crista were longer than those in the macula, while no morphological differences were noted between these two types of cells. Isolated living vestibular cells are capable of producing selfmovement. After exposure to a medium containing high concentrations of potassium, or to a hypoosmotic (290mosm) medium, the type I cells showed tiliting of the neck portion accompanied by tilting of the hair bundle of about 15 degrees.
Given the tight and dense structure of the vestibular epithelium, the changes in shape of the isolated vestibular hair cells may lead in vivo to changes of siffness of the apical region of type I cells.
