先天性前庭機能異常マウス作製法の開発

I.子宮切開法の応用に関する基礎的研究

抄録

In the development of the model mouse with congenital vestibular disorders, the technique of "exo-utero surgery", established by Muneoka et al. (1986) was modified and applied for direct manipulation of postimplantation mouse embry-os.
We conducted a basic study on the viability of exo-utero mouse embryos (Jcl: ICR) with or without injection. Hank's solution or physiological saline was injected into the telencephalic vesicle. The days of operation were from Ell to E15. The viability of exo-utero embryos was; (1) 78% (n=9) in embryos oper-ated on Ell and sacrificed on E15 (Eli-E15); (2) E12-E15: 85% (n=20), (3) E 13-E15: 80% (n=5); (4) E12-E18: 80% (n=5); (5) E14-E18: 90% (n=10); and (6) E15-E18: 100% (n=5). The viability of injected embryos was; (1) 60% (n=5) in embryos injected on Ell and sacrificed on E15 (Ell-E15); (2) E12-E15: 90% (n=10); (3) E13-E15: 100% (n=3); (4) E12-E18: 80% (n=5); (5) E14-E18: 90% (n=10); and (6) E15-E18: 100% (n=5). These results show that this method is very useful for manipulating the developing otic vesicle, and that is a possible way of developing a novel model of congenital vestibular disorders.