蛍光法による食品中の大腸菌検出方法の検討
1992 年 9 巻 3 号 p. 171-176
詳細
1992 年 9 巻 3 号 p. 171-176
Escherichia coli in various food samples including seafood was confirmed rampidly by observing fluorescence in cultured media which was prepared by adding 4-metyl-umbelliferyl-β-D-glucuronide (MUG) to EC medium, Violet Red Bile Agar (VRB) and Tryptose-soy pepton Agar. A high false positive rate, 60.8%, was observed in seafood samples when MUG-EC medium was used, however, it was overcome by replacing MUG-EC medium with MUG-VRB agar. A few false positives and negatives were observed in cooked food samples in MUG-EC medium. It was also overcome by replacing MUG-EC medium with MUG-VRB agar. The MUG-VRB agar method was more effective than the MUG-EC method for testing food samples including seafood.
