日本食品微生物学会雑誌
Online ISSN : 1882-5982
Print ISSN : 1340-8267
ISSN-L : 1340-8267
腸管出血性大腸菌O157: H7によって実験的に汚染した野菜種子に関する研究
工藤 由起子小西 良子春日 文子伊藤 嘉典岩城 正昭斎藤 典子小沼 博隆熊谷 進
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2000 年 17 巻 3 号 p. 201-205

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It is known that vegetables can serve as vehicles of food-borne illness when they are grown from seeds contaminated with pathogens. In this study, we investigated the fate of enterohemorrhagic Escherichia coli O157: H7 in experimentally contaminated radish and komatsuna seeds, after long-term storage at 4°C. The bacteria survived for 36 weeks in radish seeds initially contaminated at 103 cfu/ml, and 20 weeks survival . observed at as low as 102 cfu/ml. On komatsuna seeds, the bacterial count decreased more rapidly . Using scanning immunoelectron microscopy, the bacteria were detected on the surface of sprouts grown from contaminated seeds, and the bacteria were also present inside the stomata . However, heat treatmentat 100&degC for 5 sec effectively eliminated the bacteria from the sprouts . These results underscore the possible health risk from contaminated vegetable seeds even after long-term storage at a low temperature, and also the importance of heat treating of harvested vegetables.

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