Universal Primers Targeting β-actin Genes of Several Fish Species

Jong-Oh Kim; So-Young Oh; Myung-Joo Oh; Toyohiko Nishizawa

doi:10.3147/jsfp.48.56

短報

Universal Primers Targeting β-actin Genes of Several Fish Species

Jong-Oh Kim, So-Young Oh, Myung-Joo Oh, Toyohiko Nishizawa

著者情報

キーワード: universal primers, qPCR, β-actin gene, fish cell line, cell counting

ジャーナルフリー

2013 年 48 巻 2 号 p. 56-58

DOI https://doi.org/10.3147/jsfp.48.56

詳細

抄録

We designed a universal primer set for quantitative polymerase chain reaction (qPCR) based on the nucleotide sequences of several fish β-actin genes for counting cell number in small amounts of fish tissue. We also evaluated primer reactivity using several established fish cell lines and blood cells from diverse fish species. A strong correlation was observed between cell numbers and Ct values among olive flounder, sevenband grouper, rock bream, rock fish, rainbow trout and fish cell lines (FHM, EPC, SSN-1, BF-2 and GF), suggesting that cell number can be counted by qPCR assay using the universal primers for a wide range of fish species.

責任著者(Corresponding author)

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