Fish Pathology
Online ISSN : 1881-7335
Print ISSN : 0388-788X
Short Communications
Mortality of Pond-cultured Litopenaeus vannamei Associated with Acute Hepatopancreatic Necrosis Disease (AHPND) and White Spot Syndrome Virus (WSSV) Infection in the Philippines
Leobert D. de la PeñaNikko Alvin R. CabillonEdgar C. AmarDemy D. CatedralRoselyn C. UseroJoseph P. Faisan, Jr.Joey I. ArboledaWilberto D. MonotillaAdelaida T. CalpeDalisay D. G. FernandezCynthia P. Saloma
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2017 Volume 52 Issue 1 Pages 38-41


Mortalities of up to 60% were observed in pond-cultured Litopenaeus vannamei in Bohol, Philippines. Histopathological examination revealed typical acute hepatopancreatic necrosis disease (AHPND) pathology. PCR test generated 1,269 bp and 230 bp amplicons confirmative for the toxin-producing AHPND strain of Vibrio parahaemolyticus among shrimp sampled from eight ponds. The same samples were subjected to PCR analyses for the presence of other viruses, namely WSSV, IHHNV, IMNV, and TSV. The samples were negative for the viruses except WSSV, which was detected after one-step PCR in six out of eight ponds. These results suggested that shrimp were infected dually with AHPND V. parahaemolyticus and WSSV.

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© 2017 The Japanese Society of Fish Pathology
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