Comparative Analysis of Plasmid DNAs from Two Strains of Photobacterium damselae subsp. piscicida Isolated from Japan and the United States

Akito Tsuchiya; Aki Nishihara; Ayumi Saeki; Yuki Teru; Takashi Aoki; Tomoya Kono; Masahiro Sakai; Jun-ichi Hikima

doi:10.3147/jsfp.55.18

Short Communication

Comparative Analysis of Plasmid DNAs from Two Strains of Photobacterium damselae subsp. piscicida Isolated from Japan and the United States

Akito Tsuchiya, Aki Nishihara, Ayumi Saeki, Yuki Teru, Takashi Aoki, Tomoya Kono, Masahiro Sakai, Jun-ichi Hikima

Author information

Akito Tsuchiya
Department of Biochemistry and Applied Bioscience, Faculty of Agriculture, University of Miyazaki
Aki Nishihara
Department of Biochemistry and Applied Bioscience, Faculty of Agriculture, University of Miyazaki
Ayumi Saeki
Department of Biochemistry and Applied Bioscience, Faculty of Agriculture, University of Miyazaki
Yuki Teru
Department of Biochemistry and Applied Bioscience, Faculty of Agriculture, University of Miyazaki
Takashi Aoki
Integrated Institute for Regulatory Science, Research Organization for Nao and Life Innovation, Waseda University
Tomoya Kono
Department of Biochemistry and Applied Bioscience, Faculty of Agriculture, University of Miyazaki
Masahiro Sakai
Department of Biochemistry and Applied Bioscience, Faculty of Agriculture, University of Miyazaki
Jun-ichi Hikima
Department of Biochemistry and Applied Bioscience, Faculty of Agriculture, University of Miyazaki

Keywords: Photobacterium damselae subsp, piscicida , plasmid, inhibitor of vertebrate lysozyme (ivy)

JOURNAL FREE ACCESS

2020 Volume 55 Issue 1 Pages 18-21

DOI https://doi.org/10.3147/jsfp.55.18

Details

Abstract

Pseudotuberculosis pathogen, Photobacterium damselae subsp. piscicida (Pdp) shows some differences in virulence between Japanese and US strains. In this study, we analyzed plasmid DNAs derived from Japanese and US strains of Pdp, and found two homologous regions in these plasmids, pOT-51443-4/p91-197-1 and pOT-51443-1/p91-197-2. All genes in the p91-197-1 from US strain were observed in the pOT-51443-4 from Japanese strain. This event might have occurred during transmission of the US plasmid to Japanese strain. Inhibitor of vertebrate lysozyme (ivy), a known pathogenicity factor was encoded in the homologous region of pOT-51443-1/p91-197-2. Thus, the genomic region containing ivy may have been horizontally transmitted between the two Pdp strains.

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