1984 Volume 19 Issue 1 Pages 17-23
The changes in blood constituents were examined in ayu, Plecoglossus altivelis experimentally inoculated and naturally infected with Aphanomyces piscicida. The values of blood and plasma constituents except for erythrocyte count and hematocrit value were determined by means of using the unikit of Rapid Blood Analyzer System (RaBA-3010).
The experimental infection was done by intramuscularly inoculating into each fish a mass of the hyphae of A. piscicida SA7610 cultured in the FME broth containing 1% of glucose. Necrosis and hemorrhage of the muscle at the site of inoculation and the swelling of kidney and spleen were seen in all fish inoculated. Some of the inoculated fish died within 3 weeks. Statistically significant differences in erythrocyte, hemoglobin, total cholesterol and GPT were found between the control fish and the inoculated fish in a week after inoculation. Levels of erythrocyte, hemoglobin, Al-P, LAP and total cholesterol were decreased after inoculation. On the other hand, levels of GPT and BUN were increased. Significant differences at 5% level between the two groups in 3 weeks post-inoculation were found in erythrocyte, hemoglobin, total cholesterol, albumin, GPT, GOT, LAP, Al-P, TTT and bilirubin. No significant differences were found in hematocrit, total protein, LDH and ZTT of ayu in the course of this experiment.
The ayu naturally infected with A. piscicida were taken a pond with an epizootic of mycotic granulomatosis. The ayu were divided into two groups, one with external signs and another without them. Statistically significant differences in hemoglobin, total cholesterol, bilirubin, LDH, Al-P, LAP, GOT and GPT were found between two groups with and without affection. No significant differences were found in total protein and BUN. In affected ayu, levels of hemoglobin, total cholesterol, bilirubin, Al-P and LAP were decreased. On the contrary LDH, GOT and GPT were increased. Significant differences in bilirubin, LDH, and Al-P were found between the ayu without affection and healthy ayu which were collected from another pond without the disease. It was suggested from this fact that changes of bilirubin, LDH and Al-P levels may be characteristics of the early stages of this disease. The levels of total protein, total cholesterol and BUN varied among fish with 3 different sites of affection, nape, trunk and caudal peduncle.
