Purification and Biochemical Characteristics of Occlusion Body of Penaeus monodon-Type Baculovirus (MBV)
1992 Volume 27 Issue 3 Pages 127-130
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1992 Volume 27 Issue 3 Pages 127-130
In the present study, occlusion bodies (OBs) of Penaeus monodon-type baculovirus (MBV) were collected from infected juvenile P. monodon and then the OBs were purified by centrifugation for 30 min at a speed of 100, 000×g using 35-65% (w/w) sucrose gradient. Analysis of polypeptides of MBV occlusion bodies showed 12 polypeptides in SDS-PAGE gel. It was also concluded that the major band with molecular mass of 62k dalton may be derived from protein of polyhedrin.
