On the Tryptophan Determination in Fimbrial Protein

Abstract

This paper concerns the tryptophan determination in the fimbrial protein, which were taken off and purified from thefimbriate cells of Shieglla fiexneri Several methods were used for the tryptophan determination, including the absorption spectra in terms of tyrosine and tryptophan content, amino acids analysis of the fimbrial protein hydrolysed with alkaline, and color-forming reaction of tryptophan (p-dimethylaminobenzaldehyde). Although qualitative measurement from the absorption spectra suggested the presence of tryptophan residues in this protein, it was not able to estimate the content of tryptophan residues by other physico-chemical methods.