Effects of Culture Conditions on Shoot growth in Tissue Culture of Fragaria ananassa

Abstract

The effects of various culture conditions on the shoot growth of Fragaria ananassa were examined. To multiply the shoots efficiently, modified MS medium (with the concentrations of NH₄NO₃, KNO₃ and CaCl₂·2H₂O halved) supplemented with 1 mg/l 4PU (N-(2-chloro-4-pyridyl)-N'-phenylurea), 30g/l sucrose and 8g/l agar was selected as the optimum medium. Multiplied shoots were transferred to liquid medium and the effect of shake culture conditions on shoot growth was examined. The results revealed that the use of halved strength modified MS medium containing 30g/l sucrose stimulated the growth of shoots in a shake culture. According to the results obtained, the use of a bioreactor containing the selected medium for the production of plantlets, and reestablishment in soil, will be a sufficient method for mass propagation of Fragaria ananassa plants.