2013 年 23 巻 1 号 p. 47-52
High hydrostatic pressure and low temperature characterize the majority of oceanic environments in terms of the volume occupied. Deep-sea organisms have adapted to survive under such extreme conditions. High pressure and low temperature exert profound physiological impacts on biological membranes, primarily resulting in tighter packing and restricting the rotational motion of acyl chains. The maintenance of appropriate membrane fluidity is crucial for life under low-temperature and high-pressure conditions. Of the spectroscopic techniques available to study membrane properties, fluorescence anisotropy measurement is a common useful method providing information on dynamic membrane properties. Recently we developed a new system that enabled fluorescence anisotropy measurement under high pressure. Using this system, we elucidated the dynamic properties of the membrane in a deep-sea bacterium o23Shewanella violaceao20 under high pressure.