Development of sensitive urinary interleukin-6 (IL-6) assay by extraction technique and ELISA method and correlation between urinary IL-6 levels and the clinical activity in patients with mesangial proliferative glomerulonephritis (Mes PGN)

Abstract

The simple extraction technique of urinary IL-6 was developed, so urinary IL-6 levels can be measured by enzyme linked immunosorbent assay (ELISA) method without the influences of non-specific reactions and/or inhibitors. The urinary IL-6 levels in active stage of MesPGN cases were higher than those of remission stage of MesPGN cases, healthy cases and other glomerular disease cases. And in remission stage of MesPGN cases, urinary IL-6 levels were same level as in healthy cases.
These data suggest that measurement of levels of urinary IL-6 is helpful for the diagnosis and evaluation of clinical activities of MesPGN.