Journal of Clinical and Experimental Hematopathology
Online ISSN : 1880-9952
Print ISSN : 1346-4280
ISSN-L : 1346-4280
Short Communication
Flow Cytometric Analysis of Kappa and Lambda Light Chain Expression in Endoscopic Biopsy Specimens before the Diagnosis of B-Cell Lymphoma
Satoko OkaKazuo MuroiKazuya SatoShin-ichiro FujiwaraIekuni OhTomohiro MatsuyamaKen OhmineTakahiro SuzukiKatsutoshi OzakiMasaki MoriTadashi NagaiNoriyoshi FukushimaNoriyoshi FukushimaAkira TanakaKeiya Ozawa
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2012 Volume 52 Issue 2 Pages 127-131

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Abstract

Forty-eight patients with gastrointestinal (GI) tract B-cell lymphoma (BCL) were analyzed retrospectively. The diagnosis was based on the histological examination of specimens obtained by endoscopic biopsy. Before the diagnosis was made, single-color flow cytometry was performed to analyze the expression of light chains and B-cell antigens including CD10 in the specimens. Restricted light chain (RLC) expression, a marker of B-cell clonality, was defined as κ and λ ratios of either more than 3.0 or less than 0.5. The specimens from 30 patients (62.5%) showed RLC expression. No RLC expression or RLC expression not examined was divided into two groups : those showing CD10 positivity in more than 20% of cells (4 patients, 8.3%) and those showing no positivity (14 patients, 29.2%). The cell number analyzed in the latter group was significantly smaller than that in the other two groups. Abnormal karyotypes were found in the specimens from 8 patients (16.7%). These results indicate that the flow cytometric analysis of endoscopic biopsy specimens is useful when BCL is suspected if an adequate number of cells are obtained. [J Clin Exp Hematopathol 52(2) : 127-131, 2012]

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© 2012 by The Japanese Society for Lymphoreticular Tissue Research
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