1958 年 18 巻 5 号 p. 461-469
Present paper deals with the studies on the bile soluble character of Brucella melitensis.
Whole cultures of strains 11M and 14M of Br. melitensis were non-sensitive to bile or phenol. Some of colonies of Br. melitensis formed on a plate were sensitive slightly to bile of phenol. When the sensitive colonies were subcultured repeatedly, especially on glycerol agar medium, their sensitivity was intensified. When this strain was subcultured on liver agar, some of colonies loses their sensitivity.
Both of the above sensitive and non-sensitive strains were identical completely in agglutination reaction, heavy metal ion reaction with 10% CuSO4 or Millon's reagent, resistance to the dyes of Huddleson, growth characteristics on Petragnani's slants, urease activity, H2S gas production, growth inhibition by DEDTC patch of Renoux, colonial appearance and etc, except their reaction to bile or bile salts. This was suggesting that they were both belong to the genus Brusella and were related as a original and a variant.
Namely, the bile soluble character of Br. melitensis discussed in this paper was not necessarily to be one of the essential characters described by Benoki. It was recognized only when bile sensitive variants were formed on glycerol agar medium by repeated subcultures of some bile non-sensitive strains.
Further discussions on the above mentioned problems will be made in the following report (Keio Igaku, 35, 668, 1958) which was done by employing Mexican strains of Br. melitensis.