Abstract
Nitrate is found in normal human urine, but not nitrite. Nitrate is reduced to nitrite by Escherichia coli, Micrococcus pyogenes and Proteus vulgaris, therefore the urine of patients infected with such bacteria turns to positive for nitrite. The procedure of nitrite test employed is very simple. The reagent prepared by mixing sulfanilic acid solution with α-naphthylamine solution is added to fresh urine. If the color of urine changed to red, nitrite is positive. The authors have applied this test in outpatient treatment as an auxiliary diagnostic method with a great convenience. The results obtained are presented in the paper decoribed above.
The urinary tract tuberculosis is suspected in the chronic cystitis patients with negative nitrite reaction, because urine reaction of patients infected with streptococci, gonococci or tubercle bacilli is negative. This reaction is not influenced by urinary protein and pH of urine as well as drugs administered.
