STUDIES ON MONOAMINE OXIDASE

Report 7. Measurement of Amine Oxidase by Oxygen Electrode

Abstract

The monoamine oxidase (MAO) activities in hog kidney were measured by means of Clark's oxygen electrode. The aparatus and techniques are summarized in the text. The electrodic technique for measurement of MAO activity is very simple and acculate compared with the Warburg's manometric technique. MAO was found to be weak in affinity to oxygen, and its reaction rate was changed according to decrease of oxygen tension in the reaction mixture. According to the above mentioned reason the MAO activities were measured as the oxygen consumption of initial few minutes of the reaction. Optimum pH of MAO in the hog kidney was 8.5, and pS curve had no peak in the region of the final concentration of 10^-2 M tyramine. Differences in the results obtained by both electrodic and manometric techniques were discussed.