Abstract
Human ceruloplasmin was investigated by immuno-electrophoresis. Results obtained were as follows.
1) Human ceruloplasmin is demonstrated on α2-globulin band by agar immunoelectrophoresis and cellulose acetate electrophoresis.
2) β-Lipoprotein was also stained by p-phenylenediamine on the plate of agar immunoelectrophoresis. The band obtained by electrophoresis plate treated with β-lipoprotein immunologically was stained by amido black and p-phenylene diamine and had oxidase activity.
3) In a case of Wilson's disease which has no ceruloplasmin in its serum, band stained by p-phenylenediamine was demonstrated on the plate, indicating stained β-lipoprotein. From this standpoint of view, it is inferred that there are several factors to influence on oxidase activity of ceruloplasmin in serum.
