EFFECT OF THE ANTI-OXIDANTS ON NEPHROPATHY INDUCED BY PUROMYCIN OF AMINONUCLEOSIDE

Abstract

The role of the reactive oxygen species (ROS) in the induction of tissue injury has been reported. The process of tissue injury by ROS depends on the reaction of ROS with unsaturated bonds in the membrane lipids of the cells followed by initiation of a chain reaction of lipid peroxidation. In this experiment, as a model of glomerular disease induced by ROS, puromycin of aminonucleoside was given to rats and the reducing effect of proteinuria was examined by giving the anti-oxidant which prevents peroxidation of the membrane lipids. In addition, to detect the injurious reaction of a hydroxyl radical generated from hydrogen peroxide catalyzed by ferrous iron, deferoxamine was injected into the rats. The rats were fed chow that contained alpha-tocopherol as an anti-oxidant ; proteinuria decreased compared to the rats bred without treatment with alpha-tocopherol. The amount of lipid peroxide of the kidney, measured by the TBA method, was higher in rats not fed alpha-tocopherol, and was also higher in rats that had proteinuria. Rats injected with deferoxamine showed heavy proteinuria compared to other proteinuric rats that had not received deferoxamine. The results suggest that the amount of peroxidation of lipids of the kidney generated by the reaction of ROS, is related to increased proteinuria, and reducing peroxidation of the membrane lipids was induced in the rats by giving an anti-oxidant in the chow. To evaluate the function of a hydroxyl radical of this model, additional detailed studies are needed.