2014 年 52 巻 Supplement 号 p. SY-74-SY-75
Information processing in the brain is formed by the interaction of multiple neurons. The simultaneous intracellular recording from multiple neurons in vivo is an essential techniques, because the intracellular recording enables us to observe not only action potentials but also postsynaptic potentials. However, multiple in vivo intracellular recording is formidable tasks for researchers. To overcome this difficulty, we developed an automated intracellular recording (AIR) system. The AIR system can automatically move an electrode in the brain, find a neuron and record its intracellular potential. We evaluated the performance of the AIR system in anesthetized mice. The success rate for one electrode was 63 % (n=11 electrodes). The average stable recording time was 56 min, and a maximum time was 193 min. Additionally, we run 6 AIR systems in parallel and succeeded in simultaneous recording from 4 neurons; 2 neurons from the primary somatosensory and 2 neurons from the secondary motor area.
