生体外における三次元神経ネットワーク構築を可能とする細胞描画技術

抄録

Recently, there are numerous studies about two-dimensional cell patterning that enables to make a cell chip. However, to simulate in vivo condition on the chip, three-dimensional cell patterning technique is required for the reconstruction of complicated cellular organization in vitro. In this study, to develop three-dimensional cell organizing technique and apply it for a neural cell chip, we focus on micro-injection method which could control the three-dimensional position of cells in hydrogel. This three-dimensional cell drawing technology was consisted of micro injector and automatic positioning stage. Using our novel device, PC12 cells were located and patterned three-dimensionally in collagen gels. The width of drawn cells, length of neurite outgrowth, and the continuity of cell-network were evaluated. As the results, PC12 cells were located and positioned three-dimensionally in the collagen gel. The cells in gel extended their neurites and connected to each other.