Validation of controllability of cell-microbubble aggregations under ultrasound exposure

Abstract

Although various cellular immune therapies have been developed, because the therapeutic cells disperse in blood flow, there is a limitation on the accumulation of the cells to the target area. Thus we propose a new method of carrying therapeutic cells using microbubbles and ultrasound, where microbubbles attach on the surface of the cells, because acoustic force to propel the cells is increased. Using microbubbles and Colon-26 cells, we succeeded to produce their aggregations. Then we verified the controllability of the aggregations under continuous ultrasound exposure. According to the exposure time and the sound pressure, we confirmed the controllability of the aggregatiions decreased.