Microbial Monitoring of Crewed Habitats in Space—Current Status and Future Perspectives

Previous space research conducted during short-term flight experiments and long-term environmental monitoring on board orbiting space stations suggests that the relationship between humans and microbes is altered in the crewed habitat in space. Both human physiology and microbial communities adapt to spaceflight. Microbial monitoring is critical to crew safety in long-duration space habitation and the sustained operation of life support systems on space transit vehicles, space stations, and surface habitats. To address this critical need, space agencies including NASA (National Aeronautics and Space Administration), ESA (European Space Agency), and JAXA (Japan Aerospace Exploration Agency) are working together to develop and implement specific measures to monitor, control, and counteract biological contamination in closed-environment systems. In this review, the current status of microbial monitoring conducted in the International Space Station (ISS) as well as the results of recent microbial spaceflight experiments have been summarized and future perspectives are discussed.

Previous space experiments conducted during short-term flights and aboard orbiting space stations suggest that the relationship between humans and microbes may be changed in space habitats (environmentally controlled closed eco systems under microgravity and elevated radiation). The pathogenicity and virulence of some bacteria, such as Salmonella enterica serovar Typhimurium, have been shown to increase under microgravity, and this has been attributed to enhancements in the formation of extracellular matrices and production of biofilms (59). The immune system responses of astronauts during spaceflight are also altered (6) possibly due to the stress associated with crewed habitats in space. Manned missions to Mars, which may be realized within the next two decades, may weaken the immune status of the crew due to long-duration missions in a confined environment and ultimately cause profound changes in the composition of the bacterial flora in the intestinal tract, nasal passages, and upper airways, resulting in an increased risk of infection. Therefore, research on the relationship between human physiology and microbial ecology in space habitats is critical for the longterm operation and sustaining engineering of a human pres ence in space (38). As outlined in the mission roadmaps of the international partners on the ISS, including NASA (National Aeronautics and Space Administration; Human Health, Life Support and Habitation Systems-Technology Area 06. http://www.nasa.gov/pdf/500436main_TA06-HHLSHS-DRAFT-Nov2010-A.pdf), ESA (European Space Agency; Towards Human Exploration of Space: a European Strategy [THESEUS] Roadmap. http://theseus.hd20.hosting. punkt.de/fileadmin/Docs/Eg_reports_roadmap/RoadMap_ web.pdf), and JAXA (Japan Aerospace Exploration Agency; Kibo Utilization Scenario toward 2020 in the field of Life Science. http://iss.jaxa.jp/en/kiboexp/scenario/pdf/life%20 science.pdf), rapid and robust environmental monitoring is required to provide essential information on microbiological safety (e.g., define correct upper and lower thresholds of microbes in the air, surface, and water) to enable continued success in longduration space habitation.
Additional research is needed to better understand the dynamics of microbial colonization under space habitat con ditions and how interactions between microbial communities, humans, and life support systems impact on both human and system performance. Contamination by microorganisms in confined environments represents a potential risk for the health of the crew as well as on-board systems (37,53). Therefore, each space agency has developed and imple mented specific measures to prevent contamination by microorganisms, continuously monitor the crewed habitat and life support systems, and develop deployable microbial control and disinfection.
In this review, we describe the current status of microbial monitoring conducted on the ISS, the results of recent micro bial spaceflight experiments, and future perspectives on international cooperation to share, standardize, and improve methods, procedures, and technology.

Sampling in space habitats
NASA has monitored the presence of microorganisms since early human spaceflight into a low Earth orbit (an altitude between 160 km and 2,000 km) including the NASA-Mir and Space Shuttle programs. Preflight surface, air, and wa ter samples are typically collected between 2 months to 10 d prior to a launch based on the sample type. Samples include surface swabs of randomly selected hardware items as well as air and surface samples from the habitable volume of the vehicle. Air, surface, and water samples are also collected on a quarterly basis with microbial enumeration being performed during flight and microbial identification on samples returned to the ground for analysis (Fig. 1, Table 1).
A swab is widely used for ground processing and on-orbit surface sampling of space vehicles (61); however, this approach requires water to wet the surface for effective microbial collection, and multiple steps are involved in the current swab sampling protocol. Therefore, new sampling devices with simplified procedures are needed. One such device uses an adhesive sheet with several improvements over swab-based methods, including a simplified procedure, no water requirement for sample collection, and improved ease of transport and storage (Fig. 2). The ability of the adhesive sheet to collect microbes from a metal plate and laptop palm rest (plastic, rough surface) was found to be equivalent to that of the swab (24). Therefore, the adhesive Fig. 1. Spaceflight surface, air, and water samples are collected and microbial colonies are enumerated during flight, while microbial identification is performed on the ground. Inflight sample collection activities include swabbing surfaces (A1), air sample collection using air sample equipment (A2), and collecting water from potable water sources (A3). Enumeration is performed on incubated samples that include contact slides from surface samples (B1), culture plates from air samples (B2), and colony growth on filter discs from water samples (B3). sheet represents an alternative device for sampling in a space habitat and has been used for surface microbial sampling in Kibo, the Japanese Experiment Module of the ISS (24).

Microbial bio-contamination control
NASA has obtained much information on the risks of microbial contamination to the crew members as well as the vehicle from spaceflight microbial environmental moni toring, and has consequently developed engineering controls and monitoring strategies to minimize detrimental microbial growth during spaceflight (39). The microbial control efforts of NASA are focused on preventing microbial growth by reducing humidity and eliminating free water, maintaining high-volume exchange and air filtration, and implementing a schedule of routine housekeeping and food monitoring. The microbial environmental acceptability limits for preflight and inflight ( Table 2) were developed through spaceflight experi ence and data collected by subject matter experts in microbial monitoring and control. These limits have focused on crew health and safety in addition to the prevention of biofouling to maintain space vehicle system integrity.
If preflight or inflight samples exceed the defined limits, remediation or mitigation steps are implemented and include repeat sample collection to verify that remediation procedures are adequate to meet environmental specifications. An example of an inflight anomaly occurred in 2004 during Expedition 9 when a suspected mold was identified on a panel front in the Functional Cargo Block (Russian Segment on ISS). It was later discovered that wet clothing and towels were placed against the fabric panel to dry, causing a wet area that allowed for microbial growth. Initial remediation efforts included altering crew hygiene practices to prevent wet items from being placed in direct contact with the fabric surface and decrease the potential for microbial growth.
The prevention of bio-contamination should include ratio nal habitat designs. For example, habitats designed to mini mize the spread of biological aerosols benefit from the development of a reliable model to disperse bio-aerosol con tamination from point or diffuse sources in the habitat. BIOSMHARS (BIOcontamination Specific Modelling in HAbitats Related to Space; http://www.biosmhars.eu/) is the first joint EU-Russia research project to address this issue. The first phase of this project aimed at developing, calibrating, and validating a mathematical model to predict the dispersion of microbial bio-aerosols in the BIOS facility (http://www.biosmhars.eu/expe/bios-3), a closed environ ment in size and concept relevant for space, so far without human activity and under Earth conditions. The long-term objective of the BIOSMHARS team is to develop a versatile and robust modeling tool to predict airborne microbial con taminant dispersion and deposition in a manned spacecraft in flight.
Microbes are intimately associated with life and biological processes on Earth and will always be present in manned space habitats. If a better understanding can be obtained on the dynamics of microbial dispersal, survival, and prolifera tion in indoor confined habitats, it will help to define better strategies to monitor, manage, and control microflora to ben efit crew health and performance.

Abundance of bacteria and fungi in the ISS
Each space agency has been continuously monitoring the abundance and diversity of bacteria and fungi in their respective modules in the ISS to better understand microbial dynamics in crewed habitats in space.
NASA previously reported microbial abundance in the ISS based on the continuous monitoring and recovery of viable cells using cultivation based methods (9). Staphylococcus,  In the United States Operating Segment of the ISS, the Water Recovery System (WRS) is a physicochemical system comprised of a Urine Processor Assembly (UPA) and Water Processor Assembly (WPA) that is designed to recycle crew member urine and humidity condensate for reuse as potable water. Direct counts by microscopic enumeration revealed 8.4 × 10 4 cells mL −1 in the humidity condensate sample, but no colony-forming cells. In contrast, 3.3 × 10 5 cells mL −1 were detected in a surface swab of the WRS waste tank, and included colony-forming bacteria and fungi recovered after a 12-d incubation on solid agar media. Based on 18S rRNA sequencing and phenotypic characterization, a fungal biofilm raft recovered from the filter was determined to be Lecythophora mutabilis. A bacterial isolate recovered from a biofouling sample of the membrane in the WRS was identi fied by 16S rRNA gene sequence data as Methylobacterium radiotolerans (unpublished data).
JAXA has also been continuously performing bacterial monitoring in Kibo ( . In this research, the relative abundance and phylogenetic affiliation of bacteria and fungi collected from the interior surfaces of Kibo were determined by quantitative PCR, PCR-DGGE, and the assembly of clone libraries. The surface of the Cell Biology Experiment Facility (CBEF; incubator), inside of the door of the CBEF, laptop palm rest, air intake, air diffuser, and handrail were selected as sampling sites. A new sampling device, the microbe collecting adhesive sheet (Fig. 2), was used in addition to the traditional swabbing method (24).
Bacterial abundance at each site, except for the air intake, was equivalent to or less than the quantification limit of quantitative PCR (cells cm −2 ) in multiple experiments con ducted in different missions (Microbe-I and Microbe-II). Meanwhile, their abundance was below the minimum detec tion limit at all sampling sites in Microbe-II' and Microbe-III. The phylogenetic affiliation of bacteria collected from the Microbe-II mission was determined, and bacteria in the phyla Actinobacteria and Firmicutes were frequently detected on the surface of the PC palm rest and handrail, which were touched frequently by astronauts. Most of these bacteria have been detected on human hands as part of the normal human skin microbiota; thus, bacterial cells may be transferred to the surface of Kibo via astronaut contact.
Staphylococci and enterococci are a part of the normal human flora and are, thus, commensal microorganisms. However, they can also be opportunistic pathogens that cause a wide range of diseases. Therefore, the antibiotic resistance of staphylococci and enterococci isolated from the ISS was determined. In a collection of ISS isolates from sampling campaigns between 2002 and 2006, twentynine Staphylococcus and Enterococcus isolates were investigated for antibiotic resistance, horizontal transfer capability, and biofilm formation (46). Resistance to one or more antibiotics was detected in 22 out of 29 (75.8%) strains examined. The most prevalent resistance determinants among the staphylo cocci were ermC, tetK, and different cat genes conferring resistance to macrolide, tetracycline, and chloramphenicol, respectively (46). Plasmids were present in 86.2% of the isolates; eight of the Enterococcus faecalis isolates har bored a large plasmid of approximately 130 kb, likely to be self-transmissible. Transfer genes encoding key proteins of the conjugative transfer process, such as the ATPase delivering energy for the transfer process and the coupling protein indispensable for linking the DNA transfer and replication complex (Dtr) with the so-called mating pair formation (Mpf) complex from the well-known resistance plasmids from gram-positive bacteria such as pIP501, pRE25, pSK41, pGO1 and pT181, were detected in the total DNA of 86.2% of the strains. Most pSK41-homologous transfer genes were detected in isolates belonging to coagulasenegative staphy lococci. Twenty-eight percent of the isolates contained at least one vir signature gene, virB1, encoding a muramidase locally opening the peptidoglycan in the cell wall, virB4, encoding the motor ATPase of the plasmid transfer process, and virD4, encoding the conjugative coupling protein, respectively. Through solid surface matings, it was demon strated that several Staphylococcus spp. isolates could transfer their resistance genes to distinct Enterococcus and Staphylococcus spp. Biofilm formation was observed in 83% of the isolates (46). As methicillin-resistant Staphylococcus aureus (MRSA) or vancomycin-resistant Enterococcus (VRE) was not detected, the health risk associated with Enterococcus and Staphylococcus infections for the crew was assessed as low.
To monitor fungal microbiota in the ISS-Kibo, culturebased methods were used for swab-and flat sheet media samples in the Microbe-I experiment. Tests on orbital sam ples using either sample collection method were negative. Although adhesive sheet samples were also examined by field emissionscanning electron microscopy, no microbial cells were detected. However, fungal DNAs were detected using real-time PCR (33) and then analyzed by the clone library method. Alternaria sp. was the dominant fungal species before the launch, but the most abundant fungal species changed to Malassezia spp. on orbit. The dominant species found in ground control samples collected from the air conditioner diffuser, lab bench, door push panel, and facility surfaces in a university laboratory were Inonotus sp., Cladosporium sp., Malassezia spp., and Pezicula sp., respec tively. Malassezia spp. constitute human skin microbiota and Inonotus sp., Cladosporium sp., and Pezicula sp. are often found in natural environments such as soil and plants. These results suggested that the fungal biota in Kibo changed from soil-borne fungi to a human origin. It was concluded in 2009 that cleanliness in Kibo was equivalent to that in an ISO Class 7 clean room environment on the ground (45).
In the Microbe-II experiment, 14 strains belonging to 5 fungal species grew on board Kibo and changes in the fungal community structure were observed using clone libraries (unpublished data). In the Microbe-III experiment (September to October, 2012), 10 strains belonging to 4 fungal species were cultured from surface samples, but no fungi grew from air samples collected by an airsampling device.
A major source of the microbes found on surfaces in the ISS is thought to have been the human skin microbiota. The human skin microbiota includes various types of micro organisms, such as viruses, bacteria, and fungi. The body sites involved include sebaceous or oily sites, moist sites, and dry sites. The predominant bacterial species differ at each body site. For example, Actinobacteria, including Corynebacterium and Propionibacterium species, predo minate at sebaceous or oily sites, glabellar creases, and canals (22). Regarding the fungal microbiota, Malassezia is the predominant group at all body sites, although a few hundred fungi exist on human skin (20,48). Therefore, the fungal microbiota differs from the bacterial microbiota.
The fungal microbiota of 10 astronauts who spent time on the ISS was analyzed by quantitative PCR. Skin samples were collected from the astronauts once during the pre-flight stage, twice during the in-flight stage, and once during the post-flight stage. The astronauts stayed on the ISS for 6 months. On the ISS, the colonization levels of Malassezia on the astronauts increased, but decreased in post-flight (Fig. 3) (unpublished data). A few hundred thousand high-quality reads were obtained by pyrosequencing, and more than100 fungal genera were detected in the samples collected from the 10 astronauts; Cladosporium and Malassezia were predomi nant, followed by Aspergillus and Cryptococcus. The fungal diversity of samples collected in-flight decreased while the fungal diversity of post-flight samples increased. The fungal microbiota sampled from both environmental surfaces in the ISS and skin of crew members revealed an increase in the proportion of Malassezia species. In cheek samples, Malassezia accounted for 95% of the overall fungal species in the in-flight samples, whereas it comprised only 50 and 60% of the pre-and post-flight samples, respectively (unpub lished data). The level of microbial colonization markedly increased when access to bathing facilities was limited over a long period of time and the skin microbiota of astronauts may have changed for that reason.
From microbial abundance and their phylogenetic affiliation, the Kibo module has been microbiologically well maintained. However, increases were reported in bacterial and fungal numbers in previous space stations due to longduration habitations (37), and microbial abundance in Kibo may also increase with prolonged exposure to astro nauts. Continuous bacterial monitoring in Kibo is required to ensure crew safety and better understanding of microbial dynamics in space habitation environments.

Microbial responses to microgravity
The short generation time of microorganisms makes them uniquely suited for studies to assess responses to altered environmental conditions. Therefore, microbial cells were among the first Earth-based life forms to be sent into the environment of space. Early studies revealed that the space flight environment did not affect the viability of microorganisms (28,63,64). However, by assessing Escherichia coli on several Vostok (Russian spacecraft) flights, these initial spaceflight studies documented an increase in the number of phage particles produced, as well as a variant colony type of the bacteria, which were concluded to be the result of spaceflight factors (28,63,64). The studies that followed included assessments of basic microbial character istics, such as alterations in cell density as a result of space flight cultures. Over the course of several flight experiments, S. Typhimurium, E. coli, Candida albicans, and Bacillus subtilis displayed greater growth profiles in spaceflight than ground controls (12,16,25,26,49).
With evidence that bacteria were able to sense and respond to the microgravity environment of spaceflight, the concern of spaceflight researchers shifted to how these variations could impact on human health. Over the course of numerous spaceflights, researchers from various countries analyzed changes in antibiotic resistance by E. coli and S. aureus (51). Specifically, the minimal inhibitory concentrations (MIC) of oxacillin, chloramphenicol, and erythromycin for S. aureus and colistin and kanamycin for E. coli were evaluated among in-flight cultures and compared to ground controls. These studies reported an increase in bacterial resistance to all antibiotics tested for both S. aureus and E. coli. Additionally, the researchers observed a thickening of the cell wall that accompanied the increase in the antibiotic resistance of S. aureus once returned from flight (51).
To further define the impact of spaceflight culture on the pathogenesis of microorganisms, researchers again turned to S. Typhimurium, launching it into orbit, allowing it to grow, and returning it for assessments in a murine model of salmo nellosis (59). Mice infected with bacteria cultured in-flight displayed a decreased timetodeath, increased percent mor tality, and decrease in the lethal dose required to kill 50% of the mice (LD50) (59). In addition, samples that were fixed in-flight and analyzed on the ground revealed the differential expression of 167 genes and 73 proteins, which led to the identification of a possible role for the regulatory protein, Hfq, in the bacterial response to cultures in the spaceflight environment (59). This was the first study to elucidate both the molecular response connected with a regulatory mecha nism and alterations in bacterial virulence as a consequence of growth in spaceflight. A follow-up investigation with S. Typhimurium confirmed these virulence findings and determined that the media ion concentration influenced the spaceflight-related virulence response in this organism (60). The transcriptional and proteomic responses of spaceflight cultured Pseudomonas aeruginosa have recently been docu mented, and the involvement of Hfq and the Hfq regulon was noted (15). These studies on the involvement of Hfq represent the first account of a common molecular regulatory mecha nism, shared among different bacterial species, in response to the spaceflight environment. Although inroads into the mechanism(s) behind microbial adaptations have been made using spaceflight as a platform, and even though there have been more than 100 spaceflight experiments involving micro organisms during the past 50 years, our knowledge of how their response to this environmental parameter impacts their ability to cope with antibiotics and other environmental stresses has largely been generated through investigations utilizing a spaceflight analog cell culture system.
Continuous microgravity conditions cannot be created on Earth; however, aspects of the microgravity environment can be mimicked using ground-based simulators. The rotatingwall vessel (RWV) bioreactor has been increasingly used to enhance our understanding of microbial responses that may be occurring during spaceflight (8,18,30,(34)(35)(36). The RWV (Fig. 4A) is an optimized form of suspension culture in which cells are grown under physiologically relevant low-fluidshear conditions. A cell in liquid media in microgravity is known to experience two unique aspects that are important for modeling this environment: 1) remaining in a constant state of suspension and 2) experiencing a quiescent surrounding, devoid of shearing, turbulent forces. It is these aspects of the microgravity culture environment that the RWV bioreactor effectively simulates. The components of the RWV bioreactor system include the vessel, rotation base unit with an oxygen pump, and power supply. The vessel is a thin, cylindrical disc to which the cell culture media is introduced by a syringe via ports on the face of the vessel. Once attached to the base unit, the power supply initiates rotation of the vessel and provides a supply of oxygen through a gas permeable membrane on the inner backside of the vessel. The entire system can be housed in an incubator to allow for optimal cell growth at a fixed temperature (Fig. 4B). As the rotation of the fully filled vessel increases, its rotational velocity is transferred radially inward until rela tive fluid motion ceases (Fig. 4C), at which point the solid body rotation of the fluid is achieved (27). A cell within this environment experiences the sedimentation effect imparted by gravity. As it begins to fall toward the bottom of the vessel ("settle out"), it is carried back upward by the solid body rotation of the media and, thus, remains suspended in the fluid in an orbital path (Fig. 4D), modeling the first aspect of the microgravity environment described above. Therefore, when cultured in the RWV bioreactor, a microorganism experi ences a quiescent, lowshear, lowturbulent environment that is devoid of shearing forces and, thus, is analogous to the second aspect of spaceflight described above. As it is import ant to note the low-shear effects of the fluid on cells, the term Low-Shear Modeled Microgravity (LSMMG) has been adopted for use to accurately describe the environment pro duced by the RWV bioreactor (57). Through the study of numerous microorganisms cultured within the RWV bioreactor, similarities have been noted to both spaceflight responses and among organisms (36). For example, scanning electron microscopy images revealed an unidentified extracellular matrix around S. Typhimurium cells following spaceflight cultures (59). Additionally, bio film formation in P. aeruginosa was documented during growth in spaceflight (32). In response to the modeled micro gravity conditions within the RWV bioreactor, biofilm for mation by P. aeruginosa, S. aureus, E. coli, and C. albicans was increased (8,14,31,47). This increase in biofilm forma tion has been correlated with an increase in resistance by S. aureus, E. coli, and C. albicans. Another significant com monality between organisms is changes in responses to envi ronmental stressors following cultivation within the RWV. In response to a challenge with osmotic stress, S. Typhimurium and E. coli each displayed increased resistance following cultivation within the RWV (31,58). Furthermore, S. Typhimurium, E. coli, and P. aeruginosa all exhibited an increased ability to survive thermal conditions post-RWV culture (2,14,31,58). However, increased sur vivability to environmental stress has not always correlated to RWV cul ture conditions. While E. coli and P. aeruginosa are better able to withstand oxidative stress, S. Typhimurium and S. aureus were found to be more sensitive to this stressor fol lowing growth within the RWV (2,8,14,58). A summary of  (39) certain bacterial, fungal, and archaeal responses to the simu lated microgravity conditions within the RWV bioreactors can be found in Table 3.
Microgravity is merely one unique environmental parameter of the spaceflight environment. Investigations assessing the responses of microorganisms to additional factors, such as radiation and the vacuum of space, have been conducted in space and through simulated laboratory experi mentation (23). The findings of these studies showed that organisms and bacterial spores were able to withstand the extremes associated with exposure to the environment of space (23). Specifically, the bipolar lichen species, Rhizocarpon geographicum and Xanthoria elegans isolated at above 2,000 m from the mountains of Spain, were able to survive 16 d of exposure to radiation and the vacuum of space (44). Assessments of the survival and DNA repair mechanism(s) utilized by microorganisms to withstand these extremes are ongoing. A thorough discussion of microbial responses to the numerous environmental parameters associated with the spaceflight environment can be found within the review by Horneck et al. (23).

New methods for microbial monitoring in space habitats
As mentioned above, the cleanliness of the spacecraft and crewed environments has been a focus of each space agency since their inception. The historical records of microbial monitoring of the air, water, and surfaces of the spacecraft have provided an insight into the diversity and abundance of microorganisms within the spacecraft environment.
The current methods of bacterial and fungal monitoring on the ISS depend on the culturing of microorganisms during spaceflight and subsequent ground-based identification. This approach requires substantial crew time and uses perishable consumables that require frequent resupply because of short shelf lives. These resource requirements in combination with sample collection and analytical constraints limit rapid responses to microbial contamination when detected. Furthermore, sample return and ground-based identification will not be an option during future long-term missions. Previous environmental monitoring using traditional culture based monitoring methods has resulted in an understanding of the culturable microbiota of the spacecraft, but this only represents a small percentage of the extant microbial commu nity. As we move farther away from Earth and deeper into the frontier of space, the development of new microbial monitoring technologies aimed at detecting, quantifying, and identifying the presence of target organisms of interest will become increasingly important.
As human spaceflight moves beyond a low-Earth orbit for extended periods of time, the ability to monitor microbial contamination in-flight is just as critical as the need to rapidly detect, diagnose, and treat infectious diseases. NASA is currently working to define existing and develop new micro bial detection methods that will provide spaceflight crews with a rapid, simple, and an autonomous means of detecting microorganisms in the spaceflight environment, thereby mitigating microbial risks to both crew and craft. Subject matter experts from industry and academia, in cooperation with NASA microbiologists, generated a list of recommen-dations for long-duration mission microbial monitoring during a 2011 workshop at the NASA Johnson Space Center. The consensus of the group was that NASA should investi gate and implement a molecular-based form of microbial detection, such as real-time PCR, that could be performed in-flight. Moving forward, NASA scientists initiated a com prehensive market survey of commercially available realtime PCR platforms. In order to be considered for flight evaluation, the technology must meet multiple design criteria: 1. Use quantitative or semi-quantitative PCR technology with multiplexing abilities; 2. Have a low limit of detection (≤ 400 cells sample −1 in a 100 µL sample volume); 3. Use reagents that are shelf-stable at room temperature for a minimum of six months; 4. Provide a rapid assessment of the microbial environment on the ISS; 5. Expand monitoring of microorganisms beyond current means; 6. Reduce the frequency of sampling events; 7. Allow for crew autonomy during sample analysis; and 8. Have the ability to function in a microgravity environ ment.
Three platforms were selected for testing: the Cepheid SmartCycler, BioFire Diagnostic (formerly Idaho Technologies, Inc.) RAZOR EX, and iCubate 2.0 systems. Testing is ongoing and these instruments are being validated to assess their ability to detect target pathogens in potable water. The RAZOR EX and iCubate 2.0 systems have demonstrated the ability to meet low detection limits using a sampletoanswer methodology. To fairly identify and provide a screening pro cess for additional hardware that may be able to meet the needs of microbial monitoring during flight, a testing matrix was developed during initial testing for further evaluations of existing commercial technology and the identification of additional requirements to be used during a competitive pro posal process.
In addition to molecular-based identification methods, NASA is also investigating simple portable systems for microbial monitoring. For example, technology utilizing gold nanoparticles for the detection of medically significant micro organisms is of interest because gold nanoparticles have sev eral properties, e.g. high stability, low toxicity, and photonic properties, that support their use in biodetection applications for crewed habitats in space (50). The absorption of light is correlated to nanoparticle size, and therefore, the reflected color of the nanoparticles changes as a result of the size of the particle and/or aggregation of particles. In addition to their optical properties, gold nanoparticles can be functionalized by the covalent attachment of various biomolecules. The functionalization process has provided targeted delivery of these nanoparticles to numerous cell types, increased their detection by bioimaging, and improved their utility for gene and drug delivery for multiple therapeutic and diagnostic applications (10). The sensitivity and specificity of the assay is dependent on the use of different ligands (i.e., passivating molecules) on the surface of the gold clusters. While the physical chemical nature of the passivating layer is normally to simply stabilize the gold core, an appropriate modification of this layer can lead to "sensing" of particular functional groups on the target. Therefore, gold nanoparticles function alized by the conjugation of a ligand can serve as chromo genic biosensors in which binding of the target to the ligand results in the agglomeration of nanoparticles and a red-topurple shift.
In proof-of-concept studies of the gold nanoparticle approach, NASA researchers are developing a biodetection system to detect S. aureus, a common infectious agent that has been repeatedly isolated aboard the ISS (10,40). Gold nanoparticles have been modified by the covalent attachment of an antibody that has a high binding affinity for a protein on the cell wall of S. aureus. Data from preliminary testing of the gold nanoparticle biodetection system has shown that S. aureus could be detected in a sample in as quickly as 10 min. The system, as constructed, was specific to S. aureus, as other bacteria assessed during the studies were not bound by the nanoparticles, and the binding of S. aureus was not affected by the presence of other microorganisms in the sample. These initial investigations have shown that a simple system capable of rapidly detecting a low-density target pathogen in a complex environment using simple to interpret colorimetric indication is feasible. The development and validation of this system is ongoing.
Culture-independent techniques using direct fluorescent staining are also effective for the rapid detection of microbes. However, these methods are often labor intensive and cell concentration steps or large sample volumes are required to obtain reliable results when samples contain low numbers of target bacteria. Flow cytometry is an effective alternative to fluorescence microscopy because the procedure is rapid and sensitive. However, flow cytometers are often complex and sometimes require skilled operators for their operation, main tenance, and analytical interpretation. Therefore, a simpler and smaller system should be more useful for "on-board" counting of targeted microorganisms in a space habitat.
Microfluidic systems that use microfluidic channels (e.g., microchips [ Fig. 5]) or capillaries are powerful tools for "on-chip" fluorescent staining and enumeration of targeted microbial cells in any sample that can be fluidized (62).
Microchip-based analyses are faster, can be performed on very small sample scales, and consume less sample and reagent volumes than conventional approaches (5). Furthermore, microfluidic devices can reduce the biohazard risk because cells are analyzed in a closed system using dis posable components or devices that can be easily cleaned of contaminants and sterilized after each use. Therefore, microfluidic devices in various forms have been used to miniaturize flow cytometers (on-chip flow cytometry [19,21,43,56]). However, most of these microfluidic devices were developed to entrap or analyze the characteristics of targeted cells rather than to determine cell numbers by a simple pro cedure. A new microfluidic system was recently developed to count total bacteria and harmful bacteria without extensive sample preparation steps such as sample concentration or the pre-staining of bacterial cells (Fig. 5).
This new system has been applied to count bacterial cells in potable water. Bacteria in potable water samples were stained "on-chip" with the DNA-staining dye (4',6-diamidino-2-phenylindole [DAPI]) and counted in a microfluidic system for direct comparisons against bacterial enumeration deter mined by fluorescence microscopy (62). The ratios of the microfluidic counts to microscopic counts were 73% in puri fied household tap water and 80% in groundwater samples. The microfluidic system can detect bacterial cells in potable freshwater within one hour and the system enables the rapid detection of significant increases in bacterial numbers in water samples, a critical requirement for the routine evalua tion of microbiological water quality on orbit.
JAXA is now developing a portable microfluidic system for "real-time" and "on-board" microbial monitoring. This system will contribute to safe and sustainable long-duration space habitation. In addition, these rapid microbiological systems will not require the cultivation of potentially patho genic microbes in the closed ecological system of the space craft and will enable the rapid generation of highly accurate results. In addition to their application in space, these meth ods can be used in various industries such as pharmaceutical manufacturing, food production, and as point-of-care diag nostic techniques in clinical settings.

Future perspective of "environmental microbiology in crewed habitats in space"
As defined by the roadmaps of each space agency, indoor environmental quality control is critical in order to reduce potential hazards for the crew and vehicle. On-going microbiological monitoring in the ISS must be continued, improved, and expanded to accumulate original data on microbial dynamics in the space station environment, and microbial spaceflight experiments to predict microbial dynamics in crewed habitats in space should be promoted. Evaluating data on microbial community dynamics in the ISS can be promoted by formally sharing currently collected information among the international partners, then defining and verifying a common standardization of the protocols for microbiological monitoring in the space station environment for future monitoring. With better data sharing and protocol standardization, we can define upper and lower thresholds for the indoor environmental quality control of air, water, and surfaces in space habitats.