Abstract
Although cytoskeletal actin fiber structure is considered as important candidate for the component in mechanotransduction mechanism, still the detail of the mechanism is not clearly understood. In order to investigate the characteristics of osteoblastic response to mechanical stimuli, we have conducted the observation that focused on intracellular calcium signaling response to mechanical stimulus using microneedle for applying deformation to a single osteoblastic cell. In this study, to clarify the role of actin fiber structure in osteoblastic response to mechanical stimuli, we investigated the characteristic of cellular response to mechanical stimuli in osteoblastic cells with controlled amount of actin fiber structure. As a result, osteoblastic cells responded more sensitively to mechanical stimuli with increasing the amount of actin fiber structure. This result suggests cytoskeletal actin fiber plays the role that transmitting or amplifying deformation and force in mechanotransduction mechanism.
