Recently, there are many cell operating researches to measure cell functions in microTAS field. We have proposed a cell trapping device having micro-channels, micro-orifices and tapered structures for single cell analysis, and fabricated the prototype by multidirectional UV lithography in short process time. In this study, we designed dimensions of the tapered structure to adjust number of trapping cells. Then, we evaluated cell trapping efficiency and number of trapped cells by trap tests of fluorescent micro-beads and plant cells.