Abstract
This paper presents a novel culture technique for deriving and separating extracellular vesicles (EVs) from human lymphocytes using chemical stimulation in a microhole array devise. Lymphocytes stained with fluorescent dye DiI were immobilized on the microholes of 4 μm in diameter using cell-anchoring molecules, and stimulated chemically with sodium butyrate (NaB). EVs were derived from the cell membranes located on the microholes because flexibility of the cell membranes increases when oleyl groups of the cell-anchoring molecules are inserted into the membranes. Then, EVs passed through the microholes, which resulted in separation of EVs from lymphocytes. Also, it was found that the distribution of diameter of the obtained EVs had a peak of about 2 μm.
