多チャネルパッチクランプを目的とした微小流体デバイスの作製および評価

抄録

This paper presents a novel microfluidic device for high throughput ion-channel analysis. To analyze the electrophysiological properties of cells efficiently, we designed the electrically independent microchannels on the microfluidic device. The microfluidic device is composed of a silicon-on-insulator (SOI) layer having the trap holes with 9 or 12 μm diameter, poly-dimethylsiloxane (PDMS) microchannel chips and glass substrate with Pt electrodes. We successfully trapped the multiple cells on one device without damage by applying negative pressure to the microchannels with a syringe pump. Finally, we confirmed that the each trap hole was strongly sealed with the cell by measuring the electrical impedance, which was more than 1 GΩ, namely gigaseal.