Development and evaluation of film-stack reaction field with a micro-pillars array for micro bio-analysis

Abstract

A film-stack reaction field with a micro-pillars array using a motor stirrer was developed for the high sensitivity and rapid reaction in micro bio-analysis. The effects of the incubation time of a protein (30 s, 5 min, and 10 min) on the fluorescence intensity in Enzyme-linked immunoassays (ELISAs) were investigated using a reaction field with different micro-pillars array dimensions (5-μm, 10-μm and 50-μm gaps between micro pillars). The difference in fluorescence intensity between the well with the reaction field of 50-μm gap for the incubation time of 30 s and the well without the reaction field with for incubation time of 10 min was 6%. The trend of the fluorescence intensity in the gap between micro pillars in the film-stack reaction field was different between the short incubation time and the long incubation time because of different factors related with the biomolecule transport in each incubation times.