Abstract
This paper describes a human thrombin DNA aptamer modified acrylamide gel for biochemical sensor. DNA aptamer is single strand DNA that bind to a specific target molecule. As a preparation for fabricating the gel sensor, we fabricated ssDNA modified acrylamide gel. We used fluorescently tagged ssDNA sample solution for testing the reaction selectivity of our fabricated DNA-modified gel. We observed that only the sample of fluorescent ssDNA that has complementary to human thrombin aptamer sequence immobilized in the gel. This result shows that human thrombin aptamer DNA is properly modified into acrylamide polymer chain. We believe that our approach could be effective to construct aptamer-based biochemical hydrogel sensors.
