エリソルビン酸とL-アスコルビン酸との分離定量法について

抄録

The separation of erythorbic acid and L-ascorbic acid was successfully made by the application of descending paper chromatography with the paper of 5×60cm, which was pretreated with 2% HPO₃. A solvent of acetonitrile system (CH₃CN: CH₃COCH₃: CH₃COOH: H₂O as 80: 5: 1.2: 15) was useful as the mobile phase.After arrival of the solvent to the lower edge of the paper, it was allowed to run over for 1 or 2 hours.By this method, as an example, Rf values of 0.56 for erythorbic acid and 0.49 for L-ascorbic acid were obtained. When each 10γ of these acids were charged on the paper, the distance between the center of the two spots was about 40mm.
Following the identification with 0.02% 2, 6-dichlorophenolindophenol, the developed spots were cut off and the acids were assayed by the 2, 4-dinitrophenylhydrazine method.
The determination of these two acids added in foods was also done.The recoveries were 95.0-96.5% for erythorbic acid and 99.0-102% for L-ascorbic acid.