Eiyo To Shokuryo
Online ISSN : 1883-8863
ISSN-L : 0021-5376
Partial Purification of Tyrosinase Inhibitors in Cow Liver
Hitomi NAKAGAWA
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1974 Volume 27 Issue 4 Pages 169-173

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Abstract
Tyrosinase inhibitors in cow liver were extracted with 0.25 M sucrose containing 10-3M ethylendiamine tetraacetic acid. Subsequently, they were partially purified by centrifugation (105, 000 X g, 60min), heat treatment (100°C, 10 min) and gel-filtration with a Sephadex G-25 column. The propertiesof the partially purified tyrosinase inhibitors were studied and the following results were obtained : 1. Molecular weight of a tyrosinase inhibitor in cow liver was estimated to be less than 5, 000. 2. This tyrosinase inhibitory activity was stable against the heat treatment and unaffected by treatment with p-chloromercuri benzoic acid.
3. The inhibitor showed the maximum absorption at about 260 mμ and the minimum absorption at 225 mμ.
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© Japanese Society of Nutrition and Food Science
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