Abstract
A high speed liquid chromatographic (HSLC) with a spectrofluorometer method was applied for the determination of α-, β-, γ-, δ-tocopherols in vegetable oils.
It was found that tocopherols in vegetable oils were almost present in free forms. Vegetable oils in solvent, therefore, were possible to inject directly onto the column without any pretreatment.
Experimental procedure was as follows: Samples of oils dissolved in n-hexane were injected directly onto the column, which was a normal phase partition column, JASCOPACK WC-03. Separation of tocopherols was carried out by employing a mixture of diisopropylether and n-hexane (2: 98, v/v) as the mobile phase. The eluant was monitored continuously by a spectrofluorometer. The recovery of tocopherols added into soybean oil was approximately 103, 98, 102, and 96% for α-, β-, γ-, and δ-tocopherols, respectively.
The HSLC method have found to offer more rapid, simple, and accurate technique than conventional methods for determining α-, β-, γ-, and δ-tocopherols in vegetable oils.
