結晶化リン酸カルシウムと非晶質リン酸カルシウムのアルカリフォスファターゼへの影響

抄録

The dissolution of Ca ion from amorphous calcium phosphate of spherical 35 μm and 50 μm (35 AP and 50 AP) and high pH crystallized calcium phosphate of spherical 35 μm and 50 μm (35 BC and 50 BC), and pH changes in solutions in which both AP and BC were separately soaked, were continuously measured over time. ALPase activity of dissolved solutions from AP and BC, using osteoblast-like cells, was also investigated. The following conclusions were drawn.
1. Relationship between amount of Ca ion and pH value was not clear.
2. The amount of dissolved Ca from 35 AP and 35 BC was larger than that from 50 AP and 50 BC, because of the larger ratio of area to weight: 35 μm versus 50 μm. The amount of dissolved Ca from amorphous calcium phosphate was larger than that from crystallized calcium phosphate.
3. As to the ALPase activity, the culture solution of 35 BC was higher than the reference group after 3 hours, and the culture solution of 35 AP was higher than the reference group after 3, 6, and 48 hours.