2005 Volume 18 Issue 2 Pages 215-221
To analyse the mechanism of apical epithelialization at the implant/tissue interface, the cell contact and adhesive strength of epithelial like cells (ELC) and fibroblastic cells (FLC) derived from human gingival tissue to evaporated titanium film were investigated under four different medium conditions containing plaque extracts:no filtered (original plaque extract), 5.0 μ m-Millipore filtered, 0.45 μm-Millipore filtered, and 0.05 μm-Millipore filtered.
The plaque extracts had a greater effect upon decreasing the growth rate of the FLC cells compared to the ELC. The ELC exhibited greater adhesive strength compared to FLC in the medium with four plaque extracts. These differences in the cellular responses of both cells to the plaque extracts were also observed in their morphological changes by using phase contrast microscopy.
The 0.05 μm-Millipore filtered plaque had no decreasing effect upon the growth rate and adhesive strength, although the no filtered, 5.0 μm-Millipore filtered and 0.45 μm-Millipore filtered plaque extracts significantly demonstrated clear evidence of the decreasing effects. It was thus revealed that apical epithelialization develops by symbiosis of microorganisms including virus and both cells.
