体力科学
Online ISSN : 1881-4751
Print ISSN : 0039-906X
ISSN-L : 0039-906X
原著
患者腫瘍組織移植マウスで見られる骨格筋萎縮ではLC3陽性顆粒が増加する
安納 弘道土橋 悠田村 直輝植村 武文和栗 聡
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2018 年 67 巻 1 号 p. 99-105

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Loss of muscle tissues in cancer cachexia has been partly attributed to the activation of autophagy; however, because the experimental animal models involved only canonical cell lines, this conclusion cannot be confirmed until it is evaluated for different pathological conditions. Hence, in the present study, we histologically examined the punctate signal for LC3, an autophagosome marker, in patient-derived xenograft (PDX) mice. When 10 PDX mice grafted with colorectal cancer tissues were examined, their body weight, muscle (gastrocnemius) weight, and area of muscle fiber were all significantly lesser than those of control mice. In addition, immunofluorescence microscopy revealed that the number of LC3-positive puncta per muscle fiber or fiber area was significantly higher in the PDX mice than in control mice. These results indicate that the autophagy-lysosomal degradation system is involved in cancer cachexia-induced muscle wasting, and that PDX mice are a useful model for pathological analyses of cachexic muscle loss.

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© 2018 一般社団法人日本体力医学会
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