The Difference of Heavy Metal Accumulation and Protein Pattern between Chlamydomonas acidophila DVB 238 and Chlamydomonas acidophila KT-1

Abstract

Acidophilic algae, Chlamydomonas acidophila DVB 238 and Chlamydomonas acidophila KT-1, had very strong heavy metal tolerance in comparison with other neutrophilic algae. To explore the possibility for use of phytoremediation, two acidophilic algae were investigated the accumulation of metal (Cd and Ni) and the protein synthesis under various metal concentration.
C. acidophila DVB 238 showed higher accumulation (13.7 μg g Cd and 2.59 μg g Ni per 10⁸ cells) than C. acidophila KT-1. Moreover Cd treatment did not induce an increase of protein in C. acidophila KT-1 but C. acidophila DVB 238. Therefore these two strains seemed to have different system for accumulating Cd. So we detected changes in the soluble protein synthesis pattern by two-dimensional polyacrylamide gel electrophoresis for C. acidophila DVB 238 cells grown under different concentrations of Cd and Ni. Some of characteristic spots were analyzed by protein sequencer and MALDI-TOF Mass.