Target protein identification using Arabidopsis full-length cDNA over-expression (FOX) lines in chemical genetics

Abstract

Identification of target protein has been an integral but rate-limiting part in chemical genetics studies. Using Arabidopsis full-length cDNA over-expression (FOX) lines and chemicals that inhibit the MEP pathway as model bleaching compounds, we developed a novel target protein screening system. With this system, we effectively identified chemical-resistant plants that over-express a target protein in the MEP pathway from the FOX plants. Moreover, chemical-resistant plants that over-express a novel UDP-glucosyltransferase, which presumably inactivates the MEP pathway inhibitor in Arabidopsis, have been identified. These results suggested that the FOX screening system can be used for target protein identification in chemical genetics studies.