The analysis of P80, the component of the regulatory factor CCM1, the master regulator of the carbon-concentrating mechanism in Chlamydomonas reinhardtii

Abstract

Aquatic photosynthetic organisms can acclimate to CO₂-limiting stress conditions by inducing the carbon-concentrating mechanism (CCM). It is not known how cells can sense the decrease of environmental CO₂ concentration leading to induce CCM. We focus on the CCM1-regulatory protein complex found in Chlamydomonas reinhardtii (Kohinata et al. 2008). From a transgenic cells harboring tagged CCM1 gene, we have isolated the CCM1-complex by affinity chromatography followed by MS analyses and identified a component of the complex. Here we will present a series of data concerning to a newly identified protein P80, which is one of the component of the CCM1 complex in Chlamydomonas cells.
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