Abstract
The cell line named 1/20-B is tolerant to low boron condition (5 μM). In these cells, activity of pectin methylesterase (PME) and gene expression of paPME1, isolated from P. alba cells, were higher than that of 1/1-B cells which required enough boron (100 μM). However characteristic and transient increase of PME activity recorded in 2 days after subculture was only accompanied with low gene expression of paPME1. The participation of other genes encoding PME was deduced.
In this study, another PME cDNA (paPME2) was isolated from 1/20-B cells 2 days after subculture by RT-PCR. This cDNA was about 1,200 bp and showed high homology in the nucleotide sequence (about 90%) with PME2 isolated from hybrid poplar. However only 75 % of homology was observed to paPME1. It was suggested that the expression of paPME2 might be caused the induction of high PME activity in 2nd day of culture period.
