Abstract
Clathrin vesicle trafficking in plant is mainly composed of three pathways from trans-Golgi network (TGN) to plasma membrane (PM) or to cellular organisms such as vacuoles, and from PM to endosome (ENDO). These pathways are significantly involved in cell growth and development as well as environmental responses. Although plant clathrin vesicle trafficking has similarities to those in animals, its cellular dynamics remains unclear. In this study, we investigated cellular behavior of plant clathrin by using fluorescent protein of clathrin. The clathrin proteins mainly localised at TGN and PM. FM4-64, a fluorescent dye for tracing endocytosis revealed that the clathrin vesicles moved from PM to ENDO marked by Ara7. In addition, RFP-clathrin fusion moved along actin filament visualised by GFP. In conclusion, plant clathrin vesicles localised at TGN, PM, and ENDO, and the vesicles were trafficked by the aid of actin filaments.
