Abstract
The micronutrient zinc is essential for all organisms because it is required as a cofactor in over 300 enzymes. On the other hand, excess zinc is also toxic to cells, presumably due to competition with other biologically important ions, which in plants ultimately leads to reduced biomass leaf chlorosis, and root inhibition. To understand the effect of excess zinc on plant cells, we have performed proteomic analysis using Arabidopsis cultured cells Deep. High concentration zinc (300μM ZnSO4) or H2O are added in cultured cells at 6 days after subculture, and plasma membrane fractions were purified using two-phase partitioning after 3 or 16 hours. Each fraction was performed shotgun analysis using LC-MS. The changed proteome by zinc treatment was analyzed using software Scafold. The amount of several proteins was changed more than 2.0 fold. We would like to discuss mechanisms of zinc tolerance in Arabidopsis.
