Abstract
Plants have several strategies to adapt to the environmental supply of phosphate. However, information about the mechanism of sensing and transporting internal or external phosphate is very limited. Since phosphate is known to move easily in a plant, we studied the moving manner of phosphate by the imaging system we developed. 32P labeled phosphate was supplied to Lotus japonicas grown under phosphate deficient condition to analyze phosphate translocation or uptake manner within the plant. As a result the different accumulation pattern of phosphate between new leaves and old leaves as well as translocation manner from leaves during seed formation was analyzed through image analysis. The expression of phosphate transporter was found to be different among the tissues with different flow of phosphate movement. The result suggested that the strategy for phosphate deficiency was different among the tissues and the analysis based on each tissue is now studied more in detail.
