Abstract
Habituation is the least understood phenomenon in plant cell culture. To dissolve this, we compared the tobacco BY-2 cells with a habituated cell line of 2B-13 derived from the BY-2 cells. In fact, the culture filtrates from 2B-13 cells caused the induction of cell division in auxin-starved non-dividing BY-2 cells. The cell division factor (CDF) from the culture filtrates of 2B-13 cells has been ascribed to a glycoprotein, while those from BY-2 cells have been also identified a kind of glycoproteins, whose molecular masses are different from the CDF from 2B-13 cells. However, one difficulty was that the amounts of these CDFs were extremely low for biochemical characterization. In this communication, we tried to purify similar CDFs in the cell lysates from the BY-2 cells. As we have succeeded in purifying and characterizing the CDFs in this fraction, these outcomes will be discussed with the previously characterized CDFs and possibly deduce the role of these CDFs in the auxin-signaling pathway.
