The purpose of this study was to examine the influence of interpersonal motivation (IM) and social interaction experiences (SIE) on negative interpersonal affect (NIA) reduction. A longitudinal study of 156 male and female university and nursing school students (mainly freshman) was conducted twice during a one-week interval in April. In the first session, the participants were asked to focus on a specific target person in order to rate IM and NIA towards that person. In the second session, participants rated [NIA] toward the same target using the same procedure as the first session. The participants were also asked to report on their frequency of social interaction. Multiple regression analysis was performed, and it was evident that the relationship with the target person was important in promoting IM. Furthermore, the results also revealed that the higher the "motive to approach," the more "incompatibility" was reduced. However, against the hypotheses, SIE showed no significant effect on any NIA reduction.