リガンドで修飾された高分子を用いた生体分子間相互作用力測定

抄録

AFM is increasingly used in biological sciences not only for imaging but also for measuring the force of interaction between bio-molecular pairs. To conduct the force measurement on various biomolecular pairs effectively, we propose a new experimental system using the complex that is composed of a ligands-functionalized polymer and its receptors. This system is expected to prevent deactivation of bio-molecules, as well as non-specific adsorption between a cantilever tip and a substrate surface, since the bio-molecules of interest are bound on the flexible polymer. In an initial trial, we applied it to measure the force of interaction between avidin and biotin using CM-cellulose as a base polymer. As a result, we got 60−160 pN interaction forces which are proportional to logarithm of force loading rate, which is consistent with the reported trends.