1984 年 5 巻 Special 号 p. 270-272
A small biosensor is required in medical field and bioindustries. It is possible to prepare a small biosensor using semiconductor as a transducer. A micro enzyme sensor has been developed for clinical analysis. The micro enzyme sensor consists of immobilized enzyme and an ion sensitive field effect transistor (ISFET) which has been developed for a pH sensor. A micro enzyme sensor for neutral lipids has been developed using immobilized lipoprotein lipase (LPL) and ISFET, LPL was immobilized on an ISFET. The chip was modified with γ-aminopropyltriethoxysilane and thin organic membrane consisting of triacetyl cellulose, 1, 8-diamino-4-aminomethyloctane and glutaraldehyde. pH in the solution was changed following the lipoprotein lipase reaction. This pH change depends on the concentration of neutral lipids. The calibration curve was linear in the range 0.5 mM-2.0 mM. Furthermore, a micro enzyme sensor for urea was also developed using immobilized urease and FET. A micro oxygen sensor (Clark type) was prepared using semiconductor technologies. The micro oxygen sensor can be used as a transducer of the biosensor.