株化細胞を用いてのエンテロウイルス分離株に関する研究

III報 臨床的に異なつた小児流行性疾患例の患者および健康児から分離されたエコーウイルス6型の性状

抄録

Echovirus type 6 was isolated on FL cell culture from patients (infants and children) with aseptic meningitis, or so-called summer fever or diarrhea, and healthy children in Gifu Prefecture in epidemic stages during a period from 1961 to 1966. It was tested for various characters in FL and MK cell culture systems.
1) The recovery rate and the virus titer determined from the cytopathic effect of isolates on FL were higher on FL cells than on MK cells.
2) Non-passage virus (original echovirus type 6 specimens produced small plaques approximately 0.5 to 2.0mm in diameter predominantly on FL cells. About 0.5 to 1.0% of them were large-plaque-producing viruses.
3) Relative efficiency was higher in the large-plaque-producing viruses than in the small-plaque-producing viruses during passages of undiluted fluid specimens of stool suspensions, throat swabs, and cerebrospinal fluids on FL cells.
4) Isolates from the 2nd fluid passages on MK cells were tested for hemagglutinating capacity, but had no capacity of agglutinating human group O erythrocytes.
5) In neutralization tests, echovirus type 6 isolated on FL cells was divided into two groups, namely, strains neutralized by 10 and 100 units of anti (D'Amori) serum, respectively.
In conclusion, no relationship was observed between any character of virus isolates and any symptom of patients from whom the virus had been recovered.