1958 年 8 巻 2 号 p. 118-120
In this communication a report is made on inoculation of dengue virus (Hawaiian strain) in one-day-old hen's egg.
The results are summarized as follows.
1) The Hawaiian strain of dengue virus has been propagated in one-day-old chick embryos. The titer of embryos harvested within a period of up to 6 days after inoculation, remained at levels of 10-3.6 (mouse adapted strain) to 10-4.5 mouse LD50 (egg successive strain).
2) The highest concentration of virus was found in one-day-old chick embryos, and five-days-old chick embryos contained the virus 10 to 23 times less per 0.02g of tissue than the former.
But, there was no difference between one-day-old chick embryos and five-days-old chick embryos in the content of virus in whole chicke mbryas.