組織培養研究
Online ISSN : 1881-3704
Print ISSN : 0912-3636
ISSN-L : 0912-3636
論文
THE EFFECTS OF ULTRAVIOLET IRRADIATION AND HYPOXIA ON EXPRESSION OF GLUTATHIONE PEROXIDASE AND GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE IN THE CULTURED RETINAL PIGMENT EPITHELIUM
Kiyoshi AKEOTomoo FUNAYAMANobuyuki HAMADAYoko AKEOTadahisa HIRAMITSUYasuhiko KOBAYASHI
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2007 年 26 巻 3 号 p. 149-157

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Glutathione peroxidase (GPX) protects the phospholipids in the biomembrane barrier from damage by the free radicals. We studied cultured bovine retinal pigmented epithelial (RPE) cells exposed to UV-A and UV-B to try to determine the expression of GPX and glyceraldehdye-3-phosphate dehydrogenase (GAPDH) that are highly sensitive to oxidation. The total cellular RNA of the RPE cells was reverse transcribed by using primers for GPX and GAPDH. The quantitative real-time RT-PCR was performed to compare the effects of UV-A and UV-B irradiation on 18S ribosomal RNA (rRNA) and GPX expression using the LightCycler system. The expression of GAPDH and GPX was decreased by hyperoxia such as 20% oxygen and UV-B irradiation using electrophoresis. The LightCycler analysis, the quantitative real-time RT-PCR, showed that UV-A exposure at a small dose induced the defense mechanism against lipid peroxidation in RPE cells. However, UV-A exposure at a large dose led to a disturbance in the defense mechanism similar to that observed with UV-B irradiation; this could have a relationship with retinal diseases caused by light damage.

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© 2007 The Japanese Tissue Culture Association
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