Vegetarian diets provide relatively large amounts of vegetable, cereals, nuts and fruits. Thus those diets have relatively low intakes of protein, saturated fatty acid, retinol and vitamin B12. The plant foods do not contain vitamin B12; in particularly, vegans may have low intakes of vitamin B12. To estimate the daily intake of vitamin B12 in Japanese woman vegans, 33 duplicate portion samples of daily diets were collected from 11 Japanese adult female vegans and their vitamin B12 were determined by microbiological assay using Lactobacillus delbrueckii subsp. lactis ATCC7830. Content of vitamin B12 in Japanese woman vegan diets were as follows: range, 0-2.1μg/day; median; 0.14μg/day. Compared with estimated average requirement and recommended dietary allowance in female aged 30 to 49 years in the Dietary References Intakes for Japanese 2010, vitamin B12 in vegan diets is quite low. This study suggested that Japanese woman vegans should have the laver and permitted to have the vitamin B12 supplement or vitamin B12-fortified vegetable for preventing a vitamin B12 deficiency.